参考文献一

    稻米种植区域在中国非常广泛，大约有3000万公顷1，占到农地面积的1/4。转基因稻米的种植不可避免地会引申出环境安全问题。绿色和平组织的研究报告称，转基因食品对人类有潜在的危害，以下为报告参考文献。

　　参考文献：

　　1.Jia, H. (2004) China ramps up efforts to commercialize GM rice. Nature Biotechnology 22(6): 642

　　2.IRRI world rice statistics. www.irri.org/science/ricestat/pdfs/Table%2002.pdf <30th October 2004>

　　3.Rice Today, September 2002. Rice Facts. Essential food for the poor. www.irri.org/publications/today/pdfs/1-2/facts1-2.pdf <28th October 2004>

　　4.Ministry of Health and Welfare, Japan (1999) Guideline for weaning (revised edition) Pediatrics International 41 (1): 115 - doi:10.1046/j.1442-200x.1999.01037.x

　　5.CIAGR Research. Areas of research: Maize (Zea mays L) www.cgiar.org/research/res_maize.html <28th October 2004>

　　6.Wilson, A. Latham, J. & Steinbrecher, R. (2004) Genome scrambling – myth or reality? Transformation-induced mutations in transgenic crop plants. EcoNexus technical report. EcoNexus, Brighton, UK.

　　7.Zhai W, Chen C, Zhu X, Chen X, Zhang D, Li X, & Zhu L. (2004) Analysis of T-DNA-XA21 loci and bacterial blight resistance effects of the transgene XA21 in transgenic rice. Theor Appl Genet 109: 534-542.

　　8.Kim SR, Lee J, Jun SH, Park S, Kang HG, Kwon S, & An G. (2003) Transgene structures in T-DNA-inserted rice plants. Plant Mol Biol 52: 761-773.

　　9.Yang, L. Ding J, Zhang C, Jia J, Weng H, Liu W, & Zhang D.

　　(2004) Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Plant Cell Rep. Published online 1st October, DOI: 10.1007/s00299-004-0881-0

　　10.Wu, D.X. Shu, Q. Y. , Wang, Z. H. , Cui, H. R. & Xia, Y. W. (2002) Quality variations in transgenic rice with a synthetic cry1Ab gene from Bacillus thuringiensis. Plant Breeding 121: 198-202.

　　11.Kohli, A., Leech, M, Vain,P. Laurie, DA & Christou P. (1998) Transgene organisation in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci 95: 7203-7208.

　　12.Kohli, A. Twyman R.M.; Abranches R.; Wegel E.; Stoger E.; & Christou P. (2003) Transgene integration, organisation and interaction in plants. Plant Mol Biol 52: 247-258.

　　13.Loc, N.T., Tinjuangjun, P., Gatehouse, A.M.R., Christou, P,. & Gatehouse, J.A. (2002) Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance. Mol Breed 9:231-244.

　　14.Codex Alimentarius Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants CAC/GL 45-2003 ftp://ftp.fao.org/es/esn/food/guide_plants_en.pdf <30th October 2004>

　　15.Shu Q-Y, Cui, H-R., Ye,G-Y., Wu, D-X., Xia, Y-W Gao,M-W.& Altosaar I (2002) Agronomic and morphological characterization of Agrobacterium-transformed Bt rice plants. Euphytica 127: 345-352.; Schuh W Nelson M.R., Bigelow D.M., Orum T.V., Orth C.E., Lynch P.T., Eyles P.S., Blackhall N.W., Jones J., Cocking E.C. & Davey M.R. (1993) The phenotypic characterisation of R2 generation transgenic rice plants under field conditions. Plant Sci 89: 69-79; Wu DX, Shu, Wang. QY, Cui Z H, Xia. YW. (2002) Quality variations in transgenic rice with a synthetic cry1Ab gene from Bacillus thuringiensis. Plant Breeding 121: 198-202;

　　16.Kuiper, H.. Kleter, G.A Noteborn H.P.J.M. & Kok E.J. (2001) Assessment of the food safety issues related to genetically modified foods. Plant J. 27: 503-528.

　　17.Nakase, M. Usui Y, Alvarez-Nakase AM, Adachi T, Urisu A, Nakamura R, Aoki N, Kitajima K, & Matsuda T (1998) Cereal allergens: rice-seed allergens with structural similarity to wheat and barley allergens. Allergy 53 (Suppl 46): 55-57.

　　18.Firn, D. & Jones, C.G. (1999) Secondary metabolism and the risks of GMOs. Nature 400: 13-14.

　　19.Saxena, D. & Stotzky, G. (2001) Bt corn has a higher lignin content than non-Bt corn, American Journal of Botany. 2001;88:1704-1706.

　　20.Wu, DX, Shu, QY, Wand, ZH, Cui, HR. & Xia, YW (2002) Quality variations in transgenic rice with a synthetic cry1 Ab gene from Bacillus thuringiensis. Plant Breeding 121: 198-202.

　　21.Metcalfe, DD. (2003) Introduction: what are the issues in addressing the allergic potential of genetically modified foods? Environ Health Perspect 111 (8): 1110-1113.

　　22.Evaluation of allergenicity of genetically modified foods. Report of a joint FAO/WHO Expert Consultation on Allergenicity of Foods Derived from Biotechnology 22 – 25 January 2001 www.who.int/foodsafety/publications/biotech/ec_jan2001/en/ <30th October 2004>

　　23.Codex Alimentarius Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants CAC/GL 45-2003 ftp://ftp.fao.org/es/esn/food/guide_plants_en.pdf <30th October 2004>

　　24.Zhou, Q. Chen X, Piao J, & Gu L. (2004) Study on food safety of genetically modified rice which expressed cowpea trypsin inhibitor by 90 day feeding test on rats. Wei Sheng Yan Jiu 33: 176-179; Li, Y. Piao J, Chen X, & Zhuo Q (2004) Nutrition assessment of transgenic rice. Wei Sheng Yan Jiu 33: 303-306.

　　25.Wang,Z-H,Wang, Y, Cui,H-R, Xia, Y-W., Altosaar, I & Shu Q-Y. (2002) Toxicological evaluation of transgenic rice flour with a synthetic gene Cry1Ab gene from Bacillus thuringiensis. J Sci Food Ag 82: 738-744.

　　26.Netherwood, T. Martín-Orúe, SM. O'Donnell, AG. Gockling,S. Graham, J, Mathers, J.C & Gilbert, HJ. (2004) Assessing the survival of transgenic plant DNA in the human gastrointestinal tract. NatBiotech 22: 204-209.

　　27.Heritage, J. (2004) The fate of transgenes in the human gut. Nature Biotechnology 22: 170-173.

　　28.Shu, Q.Y. Ye, G.-Y, Cui, H.-R. Cheng, X. Xiang,Y. Wu, D. Gao, M.-W. Xia, Y.-W Hu, C.. Sardana R &. Altosaar I. (2000) Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran pests. Mol. Breed. 6: 433-439; Tu, J.M. Zhang, G. Datta, K. Xu, C. He, Y. Zhang, Q. Khush, GS. & Datta SK. (2000) Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin. Nat. Biotechnol 18: 1101-1104; Ye, G.Y. Yao H.W. Shu Q.Y. Cheng X. Hu C. Xia Y.W. Gao M.W. & Altosaar I. (2003) High levels of stable resistance in transgenic rice with a cry1Ab gene from Bacillus thuringiensis Berliner to rice leaffolder (Cnaphalocrocis medinalis (Guenee) under filed conditions. Crop Prot 22:171-178. Ye GY, Shu QY, Yao HW, Cui HR, Cheng XY, Hu C, Xia YW, Gao MW, & Altosaar I. J (2001). Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers. J Econ Entomol. 2001 Feb; 94(1):271-6.

　　29.Moreno-Fierros, L. García, N. Gutiérrez,R. López-Revilla, R.Vázquez-Padrón, RI..(2000). Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice. Microbes Infect 2(8): 885-90; . Vázquez-Padrón, R.I, Moreno-Fierros, L. Neri-Bazán, L, de la Riva, G.A & López-Revilla, R. (1999). Bacillus thuringiensis Cry1Ac protoxin is a potent systemic and mucosal adjuvant. Scand J Immunol 49: 578-584; Vázquez-Padrón, R.I Moreno-Fierros, L. Neri-Bazán, L, de la Riva, G.A & López-Revilla, R. (1999). Intragastric and intraperitoneal administration of Cry1Ac protoxin from Bacillus thuringiensis induces systemic and mucosal antibody responses in mice. Life Sciences 64(21): 1897-1912; Vázquez-Padrón, R. I., Moreno-Fierros, L. Neri-Bazán. L. Martínez-Gil, A.F., de la Riva, G.A. & López-Revilla, R.. (2000). Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice. Braz J Med Biol Res 33: 147-155; Vázquez-Padrón, R. I., Gonzáles-Cabrera, J., García-Tovar, C. Neri-Bazán, L., López-Revilla, R., Hernández, M., Moreno-Fierros, L. & de la Riva.G.A. (2000). Cry1Ac protoxin from Bacillus thuringiensis sp. kurstaki HD73 binds to surface proteins in the mouse small intestine. Biochem Biophys Res Comms 271: 54-58.

　　30.Bernstein, I. L. Bernstein, J.A., Miller, M. Tierzieva, S. Bernstein, D.I., Lummus, Z. Selgrade, M.K., Doerfler. D.L. &. Seligy V.L. (1999) Immune responses in farm workers after exposure to Bacillus thuringiensis pesticides. Environ Health Perspect 107: 575-582.

　　31.Xu, D., Xue Q, McElory D, Mawal Y, Hilder VA, & Wu R (1996). Constitutive expression of a cowpea trypsin inhibitor gene Cpti, in transgenic rice plants confers resistance to two major rice insect pests. Mol Breed 2: 167-173.

　　32.Rao TR, Rao DN, Kotilingam K, & Athota RR. (2000).Isolation and characterization of allergens from the seeds of Vigna sinensis. Asian Pac J Allergy Immunol. 18(1):9-14.

　　33.Breiteneder H.,& Radauer C. (2004) A classification of plant food allergens. J Allergy Clin Immunol. 113(5):821-30; Hoffmann-Sommergruber K (2000) Plant allergens and pathogenesis-related proteins. What do they have in common? Int Arch Allergy Immunol. 122(3):155-66.

　　34.Nti, C.A. & Phahar, W.A. (1996) Cowpea inhibition of human and bovine protease activities and the effects of processing. Food Control 7: 129-133.

　　35.Pustai, A., Grant, G., Brown, D.J., Stewart, J.C., Bardoz, S., Ewen, S.W.B., Gatehouse, A.M.R., & Hilder, V. (1992) Nutritional evaluation of the trypsin (EC 3.4.21.4) inhibitor from cowpea (Vignia unguiculata Walp.) Br J Nutr 68: 763-791.

　　36.Lima, L.M. Araujo, AH., Oliveira, AS., Pereira, RA., Miranda, MRA. & Sales, MP. (2004) Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna ungiculata (L.) Wapl.) seeds. Food Control. 15: 107-110.

　　37.Chen X, Zhuo Q, Piao J, & Yang X (2004) Immunotoxicologic assessment of transgenetic rice. Wei Sheng Yan Jiu.33(1):77-80.

　　38.Kimber, I, Dearman,RJ. Penninks,AH, Knippels,LMJ., Buchanan,RB., Hammerberg, B., Jackson, HA. & Helm, RM. (2003) Assessment of protein allergenicity on the basis of immune reactivity: animal models. Environ Health Perspect 111: 1125-1130.

　　39.Cosgrove, D.J. Gilroy, S., Kao, T., Ma, H., & Schultz, JC (2000) Plant Signaling 2000. Cross Talk Among Geneticists, Physiologists, and Ecologists. Plant Physiol, 124, pp. 499-506